African Journal of Demography and Population Studies

Journal of Demography and Population Studies, Vol. 7 (3), pp. 001-006, March, 2022. © International Scholars Journals

Full Length Research Paper

Development of a high-throughput microsatellite typing approach for forensic and population genetic analysis of wild and domestic African Bovini.

B.J. Greyling^1,2*, P. Kryger^1,5, S. du Plessis², W.F. van Hooft^1,6, P. van Helden³, W.M. Getz^1,4 and A.D.S. Bastos¹

¹Mammal Research Institute, Department of Zoology and Entomology, University of Pretoria, Pretoria 0002, South Africa.

²Agricultural Research Council, Irene, South Africa.

³Division of Molecular Biology and Human Genetics, University of Stellenbosch, South Africa.

⁴Department of Environmental Science Policy and Management, University of California at Berkeley, USA.

⁵Department of Integrated Pest Management, Research Centre Flakkebjerg, Denmark

⁶Resource Ecology Group, Wageningen University, Droevendaalsesteeg 3a, building nr. 100, 6708 PB, Netherlands

Accepted 23 November, 2021

Abstract

Conservation management and forensic traceability of African buffalo and cattle rely on the timely provision of unbiased and accurate genetic information. An approach in which 17 cattle microsatellite markers are co-electrophoresed, following amplification in three core multiplex reactions was established for this purpose. Mean allelic richness per locus was 8.24 and 6.47, for buffalo and Bonsmara cattle, respectively, whilst an unbiased match probability of 6.5x10^-17 and 1.03 x 10^-16 was obtained for each. These results confirm the usefulness of this rapid, cost-effective typing approach for forensic, paternity and fine-scale genetic analyses of wild and domestic African Bovini tribe members.

Key words: African buffalo, microsatellite, forensic, traceability, typing, Bovini, Africa.