International Journal of Diseases and Disorders

International Journal of Diseases and Disorders ISSN 2329-9835 Vol. 3 (5), pp. 001-007, May, 2015. © International Scholars Journals

Full Length Research Paper

Cassava solid-state fermentation with a starter culture of Lactobacillus plantarum and Rhyzopus oryzae for cellulase production

DJOULDE DARMAN Roger^1*, ESSIA NGANG Jean-Justin² and ETOA Francois-Xavier ²

¹The Higher Institute of Sahel, University of Maroua, P. O. Box: 33 Maroua- Cameroon.

²Department of Biochemistry University of Yaoundé I- Cameroon.

Accepted 17 October, 2014

Abstract

A mixture of two microorganisms was used as starter culture, with the aims of producing cellulose from fermented cassava roots. Cellulase yields of 430 IU/g were recorded in solid-state fermentation of cassava starch with mixed culture of Lactobacillus plantarum and Rhyzopus oryzae, isolated from cassava dried chips. High cellulase activity (8.6 IU/ml) per unit volume of enzyme broth and high yields of cellulases were obtained and attributed to the growth of both strains on cellulose fraction of cassava roots. In Solid State Fermentation with Starter mix-culture (SSFS), fermentation retting was completed in two days, and significant softening was noticed. No significant softening was evidence in Solid State Sterile Fermentation (SSSF) and Liquid state fermentation (LSF).

Keywords: Cassava, cellulases, Lactobacillus plantarum, Rhyzopus oryzae, solid state fermentation, liquid state fermentation.