ISSN 2756-3340
African Journal of Infectious Diseases Research ISSN 4729-6836 Vol. 5 (12), pp. 001-005, December, 2018. © International Scholars Journals
Full Length Research Paper
Sequencing of 16S rRNA gene for identification of Staphylococcus species in water sample
Priyanka Shukla, Shipra Srivastava, Rajeev Srivastava and Anil Kumar Rawat*
Biotechnology and Bioinformatics Division, BIOBRAINZ, 566/29 J, Jai Prakash Nagar, Alambagh, Lucknow 226005, India.
Accepted 09 September, 2018
Abstract
A bacterial strain Bz19 was isolated from a water sample collected from river Gomati at the Indian city of Lucknow. We characterized the strain using 16S rRNA gene sequence. Phylogenetic analysis showed that the strain formed a monophyletic clade with members of the genus Staphylococcus. The closest phylogenetic relative was Staphylococcus arlettae with 99% 16S rRNA gene sequence similarity. It is proposed that the identified strain Bz19 be assigned as the type strain of a species of the genus Staphylococcus (Staphylococcus sp. Bz19) based on phylogenetic tree analysis together with the 16S rRNA gene sequence search in Ribosomal Database Project, small subunit rRNA and large subunit rRNA databases. The sequence was deposited in GenBank with the accession number HM488958.
Key words: 16S ribosomal RNA gene, Staphylococcus, polymerase chain reaction (PCR), phylogenetic analysis, DNA isolation, DNA sequencing.