ISSN 2736-173X
African Journal of Malaria and Tropical Diseases ISSN 4123-0981 Vol. 10 (8), pp. 001-006, August, 2022. © International Scholars Journals
Full Length Research Paper
Gas chromatography-mass spectrometry analysis and phytochemical screening of ethanolic root extract of Plumbago zeylanica, Linn.
G. O. Ajayi1*, J. A. Olagunju1, O. Ademuyiwa2 and O. C.Martins3
1Department of Medical Biochemistry, Faculty of Basic Medical Sciences, Lagos State University College of Medicine (LASUCOM), P. M. B 21266, Ikeja-Lagos, Nigeria.
2Department of Biochemistry, College of Natural Sciences (COLNAS), University of Agriculture (UNAAB), Abeokuta, Ogun State, Nigeria.
3Lagos State Drug Quality Control Laboratory, Lagos State University Teaching Hospital (LASUTH), Ikeja-Lagos, Nigeria.
Accepted 4 July, 2022
Abstract
Plumbago zeylanica is a medicinal plant widely used in forklore medicine in Africa and Asia for the management of ailments such as parasitic diseases, scabies, ulcers, piles, diarrhoea, skin diseases, leprosy, fever or malaria, rheumatism and intestinal parasites. The present study was carried out to identify the phytochemical components present in the ethanolic root extract of the plant by phytochemical screening methods and GC-MS analysis. The phytochemical screening showed the presence of alkaloids, tannins, steroids, flavonoids, saponins, anthraquinones, cardiac-glycosides, phlobatinnins and carbohydrates. The gas chromatography-mass spectrometry (GC-MS) analysis also identified the presence of phytochemical components like phenolics (Phenol, 2,4-bis(1,1-dimethylethyl) (RT: 6.796), Cyclopentadecane (RT: 7.305), fatty acids/methyl esters, Feroprofen (RT: 9.602), 1,1,1,5,7,7,7-Heptamethyl-3,3-bis(trimethylsiloxy)tetrasiloxane (RT: 8.253), Indazol-4-one, 3,6,6-trimethyl-1-phthalazin-1-yl-1,5,6,7-tetrahydro- (RT: 10.162), 2H-Indol-2-one,1-(2,6-dichlorophenyl)-1,3-dihydro- (RT:11.191), 2-Methyl-7-phenylindole (RT: 14.540), 1H-Indole-2-carboxylic acid, 6-(4-ethoxyphenyl)-3-methyl-4,5,6,7-tetrahydro-, isopropyl ester (11.528), and Anthranilic acid, 3-chloro-N-(o-chlorophenyl)- (RT:12.443).
Key words: Plumbago zeylanica, gas chromatography, mass spectrometry, phytochemical screening.