ISSN 2756-3294
African Journal of Botany ISSN: 3519-3824 Vol. 6 (1), pp. 001-008, January, 2018. © International Scholars Journals
Full Length Research Paper
Optimal conditions for maximizing production of reducing sugars from microwave-assisted FeCl3 pretreated rice straw degraded by Trichoderma viride and Bacillus pumilus
Ji-Liang Lü and Pei-Jiang Zhou*
School of Resource and Environmental Science, Hubei Biomass-Resource Chemistry and Environmental Biotechnology Key Laboratory, Wuhan University, Wuhan 430079, China.
Accepted 21 October, 2017
Abstract
Microwave-assisted FeCl3 pretreated rice straw was degraded to reducing sugars with Trichoderma viride and Bacillus pumilus. Response surface methodology (RSM) and Box-Behnken design (BBD) were used to optimize degradation conditions of pretreated rice straw with respect to initial pH, degradation temperature, T. viride/B. pumilus inoculum ratio (T/B inoculum ratio) and degradation time to achieve the highest production of reducing sugars. The maximum production of reducing sugars obtained was 7.19 g/L at the optimal conditions of initial pH, 8; degradation temperature, 32°C; T/B inoculum ratio, 3 ml/1 ml; degradation time, 60 h. This optimization strategy enhanced the production of reducing sugars from 6.62 to 7.19 g/L after optimization, which shows the beneficial effect on optimization of degradation conditions.
Key words: Microwave-assisted FeCl3 pretreated rice straw, Trichoderma viride, Bacillus pumilus, degradation conditions, response surface methodology (RSM) optimization.