International Journal of Veterinary Medicine and Animal Health

ISSN 2756-3871

International Journal of Veterinary Medicine and Animal Health Vol. 1 (1), pp. 001-004, January, 2010. © International Scholars Journals

Full Length Research Paper

A rapid method for testing the efficacy of teat dip formulations as bactericidal agents for the control of bovine mastitis

Pasquale P. Vicario*, Irina A. Grigorian and John Pascoe B. S.

Department of Life Sciences, Hydromer, Inc. Branchburg, NJ USA 08876

Accepted 21 June, 2009

Abstract

A nylon membrane-based filter technique was developed to evaluate the bactericidal efficacy of mastitis control teat dip products. Using this method, we were able to determine that this protocol was both a rapid and effective method for evaluating the efficacy of our iodine- and bronopol-containing teat dips against a variety of mastitis-causing strains of bacteria such as E. coli, S. aureus, P. aeruginosa, S. uberis, E. faecalis, and S. agalactiae, S. dysgalactiae, as well as the fungal strain C. albicans. The data presented in this report suggests that this method is useful in the initial assessment of newly developed bovine teat dips, as well as other medical formulations.

Key words: Bactericide, bovine mastitis, disinfectant teat dip.