ISSN 2756-3375
African Journal of Immunology Research ISSN 2756-3375 Vol. 10 (5), pp. 001-007, May, 2024. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full Length Research Paper
Development and Prokaryotic Expression of a Fusion Protein Combining PRRSV GP5 with Mycobacterium bovis HSP70
Huan-Rong Zhang1*, Qi-Gui Yan2,3, Rui Song2, Ting Feng2, Ya Liu2, Lei Zhou2 And Tao Lin2
1College of Life Science and Technology, Southwest University for Nationalities, Chengdu 61004, P. R. China.
2College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, P. R. China.
3Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Ya’an
625014, P. R. China.
Accepted 22 April, 2024
Abstract
Porcine reproductive and respiratory syndrome (PRRS) is an economically important swine disease that has devastated the swine industry worldwide. Vaccination with live attenuated vaccine or inactivated vaccine is the main treatment to control PRRS. However, the disadvantages such as virulence resumption of the attenuated vaccine and low immunogenicity of the inactivated vaccine call for a more efficient and safer genetically engineered vaccine. In this study, the structural protein GP5 of the PRRS virus (PRRSV), one of the major protective antigens which stimulates a protective immune response was selected to develop a genetically engineered subunit vaccine. In order to promote the immune reaction of the host to GP5, heat shock protein 70 (Hsp70) was selected as immuno-adjuvant to enhance PRRSV GP5 immunogenicity. The Hsp70 gene was amplified by PCR from attenuated Mycobacterium bovis, and the PRRSV GP5 gene was amplified by RT-PCR from the total RNA of PRRSV SCQ strain which was isolated, identified and maintained by the Animal Biotechnological Center, Sichuan Agricultural University, China. The fusion expressing plasmid pET32-GP5-Hsp70 was constructed and expressed in Escherichia coli BL21. Ni2+-chelating resin was used to purify the His-tagged fusion protein expressed under optimized expressing conditions. The rabbit anti-GP5-Hsp70 fusion protein antibody was made, and Western blot assay verified the successful expression of the fusion protein, making it possible for further investigation whether Hsp70 could improve the immunogenicity of the PRRSV GP5 subunit vaccine, or evaluating the immunogenicity of the GP5-Hsp70 subunit vaccine.
Key words: Porcine reproductive and respiratory syndrome virus (PRRSV) GP5 gene, Mycobacterium bovis Hsp70 gene, cloning, prokaryotic expression, identification.