ISSN 2756-3340
African Journal of Infectious Diseases Research ISSN 4729-6836 Vol. 5 (5), pp. 001-005, May, 2018. © International Scholars Journals
Full Length Research Paper
Isolation of proteolytic, lipolytic, and bioemulsifying bacteria for improvement of the aerobic treatment of poultry processing wastewater
Rattana Tarntip1* and Thungkao Sirichom2
1Department of Environmental Science, Faculty of Science, Burapha University, 169 Long-Hard Bangsaen Road, Saen Sook Sub-district, Mueang District, Chonburi, 20131, Thailand.
2Department of Microbiology, Faculty of Science, Burapha University, 169 Long-Hard Bangsaen Road, Saen Sook Sub-district, Mueang District, Chonburi, 20131, Thailand.
Accepted 15 January, 2018
Abstract
Proteolysis, lipolysis, as well as, emulsification play important roles for the biological treatment of poultry processing wastewater. Three isolates identified by 16S rRNA gene sequence analysis as belonging to Bacillus subtilis, Bacillus thuringiensis, and Lysinibacillus fusiformis, were then selected. The three combined strains provided a 71.2 ± 0.20% reduction of the chemical oxygen demand (COD) and produced a high biosurfactant 1250 ± 10 mg/L. Moreover, all three bacteria were able to form good biofilm. The combined bacteria strains performed well in terms of COD reduction, protease, and lipase and biosurfactant production at an initial pH of 7.0 and over a wide range (1000 to 5000 mg/L) of organic loads.
Key words: Proteolytic, lipolytic, poultry, wastewater, treatment, biosurfactant.