ISSN 2736-1594
International Journal of Obstetrics and Gynecology ISSN 2326-7234 Vol. 7 (6), pp. 001-005, June, 2019. © International Scholars Journals
Full Length Research paper
Feasibility of a nested PCR for the diagnosis of vaginal trichomoniasis: study in Al-Madinah Al-Munwarrha, Saudi Arabia
Fawzia A. Habib1, Amany A. El-Aal2*, Nabeil S Yamany3, Ahmed M. mohamadein4, Omima Hamed4, Ahmed Bahashwan5, Nabeil Shehata5 and Lamia A. El-Hosseiny6
1Department of Obstetrics and Gynecology, Faculty of Medicine, Taibah University, Saudi Arabia.
2Department of Parasitology, Faculty of Medicine, Taibah University, Saudi Arabia.
3Department of Obstetrics and Gynecology, Maternity and Children's Hospital, Al-Madina Al-Munawarah, Saudi Arabia.
4Department of Biochemistry, Faculty of Medicine, Taibah University, Saudi Arabia.
5Laboratory of Obstetrics and Gynecology, Maternity and Children's Hospital, Al-Madina Al-Munawarah, Saudi Arabia.
6Department of Biomedical Technology, National Research Center, Cairo, Egypt.
Accepted 27 April, 2019
Abstract
In the present study, three vaginal swabs were collected from 1469 females clinically suspected of having Trichomonas vaginalis (T. v) infection. All samples were screened by both wet mount and Diamond's culture media that was considered as the golden standard in this study. T. vaginalis gene detection by nested PCR using 4 primers targeting the Tv-E650 gene was performed on the preserved vaginal uncultivated samples corresponding to the culture positive vaginal specimens plus 30 randomly selected samples equaled to those obtained negative culture results. The prevalence of T. vaginalis infection among our patients was calculated according to the results of the golden standard culture method to be 1.43% (21 out of 1469). Wet preparation was positive for only 13 samples and missed 8 samples. PCR diagnosed 20 samples and missed one specimen that became positive after 4 days of cultivation. In this study, PCR for trichomonads does not appear to offer a diagnostic advantage and its sensitivity did not exceed that of culture. Successful culture of T. vaginalis requires only the multiplication of a single organism, the same as that needed for PCR. Therefore, the present work is highly recommending the use of Diamond's culture in the diagnosis of trichomoniasis in women.
Key words: Trichomonas vaginalis, nested PCR, wet mount, diamond's culture, vaginal swabs, Saudi Arabia, Al-Madina Al-Munawarah.