International Journal of Virology and Parasitology, Vol. 6 (4), pp. 001-010, April, 2017. © International Scholars Journals
Full Length Research Paper
Production and optimization of invertase from pomegranate peel by marine fungal strain aspergillus terreus
Aliaa R. El-Shamy and Sherien M.M. Atalla
Department of Chemistry of Natural and Microbial Products, National Research Centre, Giza, Egypt
Accepted 20 December, 2016
Abstract
Agricultural wastes were the low cost raw material for invertase production by fungi. The marine fungal strain Aspergillusterreus was characterized and phylogenetic analysis by 18S rRNA gene sequencing then evaluated for invertase production using 40 g/l of pomegranate peel (Pp) as substrate. Maximum invertase activity was 264.4 U/ml after 5 days incubation period. Two disks 6mm in diameter, pH 6.5 and 30 ◦C were the optimum conditions for invertase production. 3% of triton x100 as surfactant produce 281.2 U/ml .Increasing invertase production by addition of wheat bran ,EDTA and cysteine to pomegranate peel.
Keywords: marine fungi, invertase, pomegranate peel, optimization.