International Journal of Medicinal Plants Research

International Journal of Medicinal Plants Research ISSN 2169-303X Vol. 6 (1), pp. 312-317, January, 2017. © International Scholars Journals

Full Length Research Paper

Biosynthesis of silver nanoparticles using Trigonella Foenum Graecum and the determination of their antimicrobial activity

Jithesh Pooloth

Department of Biochemistry, Dr. NGP Arts and Science College Coimbatore, Bharathiar University, India.

E-mail: [email protected]. Tel.: 09446389039

Accepted 05 January, 2013

Abstract

Generally, nanoparticles are prepared by a variety of chemical methods which are not environmentally friendly. Use of plants in the synthesis of nanoparticles is quite novel leading to truly green chemistry which provided advancement over chemical and physical method as it is cost effective and environmentally-friendly. We have reported a fast, convenient and extracellular method for the synthesis of silver nanoparticles by reducing silver nitrate with the help of fenugreek seeds (Trigonella Foenum Graecum) extract. The characterization of nanoparticles was done by using UV- Vis Spectrophotometer. The morphology of silver nanoparticles was confirmed by Scanning Electron microscopy (SEM) and X Ray Diffraction Analysis (XRD). The antibacterial activity of these nanoparticles were studied against E.coli, Streptococcus Pneumonia, Proteus Vulgaris, Pseudomonas Aeruginosaand Staphylococcus Aureus  comparing with the standard drug Ampicilin.

Keywords: Silver nanoparticles, biological synthesis, fenugreek seed extract, SEM, XRD, antibacterial activity, E.coli, Streptococcus Pneumonia, Proteus Vulgaris, Pseudomonas Aeruginosa, Staphylococcus Aureus, Ampicilin curve.