ISSN 2756-3669
International Journal of Bacteriology and Mycology ISSN 2932-7521 Vol. 6 (2), pp. 001-005, February, 2018. © International Scholars Journals
Full Length Research Paper
Identification and quantification of Escherichia coli from drinking water in Bangladesh
Shahedur Rahman1*, Anowar Khasru Parvez2 and Rezwanul Islam3
1Department of Genetic Engineering and Biotechnology, Jessore Science and Technology University, Jessore -7408, Bangladesh.
2Department of Microbiology, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.
3Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia-7003, Bangladesh.
Accepted 26 October, 2017
Abstract
This study was undertaken to identify and quantify Escherichia coli from drinking water from the western part of Bangladesh. Fifty water samples were collected from different regions. The presence of E. coli was determined on the basis of gas formation in the test tubes after inoculating and incubating the water samples into the Lactose fermentation broth (LB). A certain amount of the accumulated gas was used to determine the quantity of the E. coli cell present in the water samples with the help of Minimum Probable Number (MPN) method. Then the confirmation test was done using Eosin Methylene Blue agar. Positive isolates were then subjected to the completed test (Gram staining). Among the 50 different water samples, 5 samples were found positive.
Key words: Escherichia coli, identification, minimum probable number (MPN), eosin methylene blue agar, metallic sheen, household water treatment.