ISSN 2756-3391
African Journal of Parasitology Research ISSN 2343-6549 Vol. 5 (11), pp. 360-365, November, 2018. © International Scholars Journals
Full Length Research Paper
Phenol-Chloroform-based isolation of Schistosoma haematobium DNA in urine samples at Institut Pasteur of Côte d’Ivoire
Angora KE1,3, Ako AB1, Bosson-Vanga AH1,3, Ira-Bonouman AV1, Touré OA1, Menan HE3, Djaman AJ2
1Department of Parasitology Mycology, Institut Pasteur of Côte d’Ivoire, BPV 490 Abidjan
2Département of Biochemistry, Institut Pasteur of Côte d’Ivoire, BPV 490 Abidjan
3Department of Parasitology Mycology, Faculty of Pharmacy, Felix HouphouëtBoigny University, BPV 34 Abidjan, Côte d’Ivoire.
Corresponding Author. E-mail: [email protected]
Accepted 27 September, 2018
Abstract
Schistosoma haemtobium infection remains an important place in neglected tropical diseases in sub-Saharan Africa. DNA detection of this parasite is never done in Côte d'Ivoire; so it is important to carry out this study which aims to perform phenol-chloroform method S. haematobium DNA extraction from urine in Parasitology Unit of Institut Pasteur. Thus, 107 urine samples positive for S. haematobium stored at -20°C collected from schoolchildren in Guebo 2 were used. Optical densities (OD) were measured by spectrophotometer at 260 nm and 280 nm wavelength and DNA concentrations were measured. The average of optical density was 0.780 ± 0.61 at 260 nm and 0.687 ± 0.53 at 280 nm. Most extracts DNA had OD260nm / OD280nm ratio lower than 1.8. DNA concentrations were high with an average of 234.0 ± 184.3μg / mL with extremes at 33.7μg / mL and 827.5μg / mL. Phenol-chloroform method gave relatively high concentrations of DNA. The PCR amplification of DNA will allow improving this method.
Key words: Schistosoma haematobium, DNA, Extraction, Phenol-chloroform method.