ISSN 2756-3413
African Journal of Virology Research ISSN 3421-7347 Vol. 13 (5), pp. 001-005, May, 2019. © International Scholars Journals
Full Length Research Paper
Detection of homozygosity in near isogenic Lines of non-susceptible to Zhenjiang strain of densonucleosis virus in silkworm
Li Muwang1*, Hou Chengxiang1, Zhao Yunpo3, Xu Anying1, Guo Xijie1, 3 and Huang Yongping2
1Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, P. R. China.
2Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 200032, P. R. China.
3College of biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, P. R. China.
Accepted 08 January, 2019
Abstract
The near isogenic lines (NILs) of non-susceptible to DNV-Z were bred through backcrossing successively using L10 as donor parent and Js as recurrent parent. The homozygosity of the NILs was detected using the SSR markers from the SSR linkage map. The results showed that the ratio of the linkage groups from the recurrent parent in the NILs increased rapidly along with the increase of the backcrossing generations. After 6 generations of backcrossing, it was selfed and was fed with DNV-Z. The linkage groups of 9 of the 5 BC6 pairs, whose 1/4 offspring were non-susceptible to DNV-Z, were all replaced by the recurrent parent except the linkage group that held nsd-Z, and only part of one linkage group from the other one individual had not been replaced. According to the markers linked to nsd-Z, Fl0316 had not been replaced in all of the 10 individuals, but crossover happened in 2 individuals between Fl0568 and nsd-Z.
Key words: Silkworm, Non-susceptible to DNV-Z, Near Isogenic line, SSR marker, Homozygosity and Linkage group.