African Journal of DNA and Cell Biology Vol. 1 (3) pp. 072-076, January, 2015. © International Scholars Journals
Full Length Research Paper
DNA harm and reduction of cell oxidase action in piglet sertoli cells presented to gossypol
*Zhou F. Ming , Jiang Z. Heng and Wang Guangcheng
College of Veterinary Medicine, Faculty of Medicine, Hong Kong University of Science and Technology, Kowloon, China.
E-mail: [email protected].
Accepted 5 January, 2011
Abstract
The study was designated to explore the toxic effects of gossypol on piglet sertoli cells. Sertoli cells were isolated from piglet testes using a two-step enzyme digestion and followed by differential plating. Piglet sertoli cells were cultured and classified into five groups, that is, group A, the control without gossypol, group B with 2.5 μg/ml gossypol, group C with 5 μg/ml gossypol, group D with 10 μg/ml gossypol and group E with 20 μg/ml gossypol. We found that sertoli cells’ growth was inhibited by gossypol at dose 2.5 μg/ml when compared with the control group. The oxidase activity of sertoli cell also decreased at 2.5 μg/m gossypol. Moreover, DNA damage of sertoli cells was observed at 5 μg/ml gossypol. Putting this into consideration, our study suggests that exposure of gossypol to sertoli cells leads to an inhibition of sertoli cell growth and oxidase activity of sertoli cells at a low concentration, whereas gossypol results in DNA damage of sertoli cells at a higher concentration.
Key words: Gossypol, sertoli cells, oxidase, DNA damage.