ISSN 2756-3391
African Journal of Parasitology Research ISSN 2756-3391 Vol. 7 (4), pp. 001-008, April, 2020. © International Scholars Journals
Full Length Research Paper
Optimize culture conditions for biomass and sporulation of the nematophagous fungus Pochonia chlamydosporia, isolate HSY-12-14 by “two-step” cultivation
Li Gao
State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, People’s Republic of China. E-mail: [email protected].
Accepted 17 January, 2020
Abstract
Biomass yields and sporulation of Pochonia chlamydosporia isolate HSY-12-14 was concerned on culture conditions including culture method, nutritional requirements together with environmental factors in this study. We optimized them for biomass yields of P. chlamydosporia HSY-12-14 with the “two-step” cultivation method as well as orthogonal matrix method: firstly cultured spore suspension on the basal medium (sucrose 19.00 g, soy peptone 4.06 g, K2HPO4 1.00 g, KCl 0.50 g, MgSO4 0.50 g, FeSO4 0.01 g and 17.00 g Bactor) for the first stage culture of 4 days under room condition, then transferred them to another defined medium (sucrose 19.05 g, soypeptone 0.01 g, ZnSO4 7H2O 0.05 g/L, MnSO4 H2O 0.05 g/L, H3BO4 0.05 g/L and 17.00 g Bactor) for more 4 days cultivation, together with the environmental factors combination of water potential -3.9 MPa /pH 4 /24 h light cycle/23°C for biomass yields. We optimized the best culture conditions for sporulation of P. chlamydosporia under water potential -1.2 MPa /pH 4 /24 h light cycle/32°C wit h the same nutrition for biomass yields. These results is characterized in that it supplies the composition of media and environmental suitable for the mass production and provide important information industrialization of this great potential biocontrol fungus.
Key words: Nutrition, environment, sporulation, biological control, mass selection.