ISSN 2756-3413
African Journal of Virology Research ISSN 3421-7347 Vol. 5 (6), pp. 001-007, June, 2011. © International Scholars Journals
Full Length Research paper
Analysis of bacterial strains from contaminated and non-contaminated sites for the production of biopolymers
M. Usman Arshad, Nazia Jamil*, Nighat Naheed and Shahida Hasnain
Department of Microbiology and Molecular Genetics, University of the Punjab, Quaid-e-Azam Campus, Lahore-54590, Pakistan.
Accepted 21 February, 2011
Abstract
A total 18 strains were collected from non-contaminated and contaminated environments, and were purified. All purified strains were characterized for Gram reaction and biochemical analysis. Screening for bioplastic production was done by Sudan black staining. Strains isolated from non-contaminated sites showed no polyhydroxyalkanoate (PHA) production. Biochemical analysis showed that PHA producing strains belong to Pseudomonas, Citrobacter, Enterobacter, Klebsiella, Escherichia and Bacillus genera. PHA extraction was done by sodium hypochlorite digestion method. Strain MS2D showed maximum percentage (65%). Production of PHA was optimized for different temperatures and pH. Selected strains were also tested for exopolysaccharides (EPS) production on EPS detection medium by solvent precipitation method. Four out of nine strains exhibited EPS production ability. EPS production was also optimized for different temperatures and pH.
Key words: Biopolymer, bioplastic, exopolysaccharides, contaminated environment.