International Journal of Veterinary Medicine and Animal Health

ISSN 2756-3871

International Journal of Veterinary Medicine and Animal Health Vol. 8 (5), pp. 001-003, May, 2017. © International Scholars Journals

Full Length Research Paper

Molecular detection of canine parvovirus in Jos, Nigeria

S. C. Chollom1,2, E. J. Fyaktu2, A. E. J. Okwori3, G. O. A. Agada4, G. Hashimu5, R. Y. Akele3, E. I. Voumangai6, T. Dashe7 and D. Z. Egah3

1Viral Research Department, National Veterinary Research Institute, Vom, Nigeria.

2Department of Medical Microbiology, University of Jos, Nigeria.

3Federal College of Veterinary and Medical Laboratory Technology, National Veterinary Research Institute, Vom, Nigeria.

4Central Diagnostics Laboratory, National Veterinary Research Institute, Vom, Nigeria.

5Bacterial Vaccine Production Department, National Veterinary Research Institute, Vom Nigeria.

6Laboratory Department, Plateau State Specialist Hospital, Jos, Nigeria.

7Institute of Human Virology Nigeria, Plateau State Virology Research Centre, Plateau State Specialist Hospital Jos, Nigeria.

Accepted 28 November, 2016

Abstract

Canine parvovirus (CPV) remains the most significant viral cause of enteritis in puppies over the age of two months. This study was meant to detect the virus by targeting the VP2 gene, a 583 bp gene (nucleotide 4003 to 4585) of the capsid protein. The detection of the virus was carried out by conventional polymerase chain reaction (PCR) on one hundred and nine samples. Seventy five of these were rectal swabs while thirty four were necropsy tissues, all from dogs presenting with symptoms suggestive of parvovirus enteritis from veterinary clinics in Jos metropolis. Results revealed that 47.70% of the samples were found to be positive for the virus. The rate of detection was more in necropsy tissues (64.71%) relative to rectal swabs (40.00%). A breakdown of the incidence of the infection across breeds of dogs showed that the Rottweillers had 60.00%, Doberman pinchers had 54.54%, Pitbulls had 53.33% while Tan coloured and local breeds of dogs had 50.00 and 21.42%, respectively. With a prevalence rate of 47.70%, the findings have confirmed that the virus is circulating in Jos, Nigeria. As such, stake holders must quickly intervene to arrest the situation given the high economic losses associated with the disease.

Key words: Canine parvovirus, polymerase chain reaction (PCR), Jos.

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T. Dashe and D. Z. Egah on Google Scholar T. Dashe and D. Z. Egah on Pubmed E. I. Voumangai on Google Scholar E. I. Voumangai on Pubmed E. J. Fyaktu on Google Scholar E. J. Fyaktu on Pubmed G. Hashimu on Google Scholar G. Hashimu on Pubmed G. O. A. Agada on Google Scholar G. O. A. Agada on Pubmed S. C. Chollom on Google Scholar S. C. Chollom on Pubmed R. Y. Akele on Google Scholar R. Y. Akele on Pubmed A. E. J. Okwori on Google Scholar A. E. J. Okwori on Pubmed