ISSN 2375-0715
African Journal of Fisheries Science ISSN 2375-0715 Vol. 11 (3), pp. 001-004, March, 2023. © International Scholars Journals
Full Length Research Paper
Interaction of polygalacturonase enzymes from Fusarium oxysporum with tomato polygalacturonase inhibiting protein
Ali Salehzadeh
Microbiology Department, Islamic Azad University, Rasht Branch, Rasht, Iran. E. mail: [email protected]. Tel: +989126932196.
Accepted 20 January, 2023
Abstract
Polygalacturonases (PGs) are important pectolytic enzymes produced by phytopathogenic fungi during the process of infection and colonisation of the host plants. In this work to study the inhibiting effect of polygalacturonase inhibiting protein (PGIP) on polygalacturonase (PG) enzyme from Fusarium oxysporum f.sp lycopersici, (highly virulent isolates), protein extraction was carried out from various tissues (stem, leaf and root) of 40, 60 and 80 days old tomato plants (Lycopersicon esculentum cv. FDT 202). Study of PGIP- PG interaction showed correlation between the plant age and increasing effect of inhibition activity of PGIP, with stem PGIP having the most effect. There were some differences in protein patterns of induced and non-induced tomato plants by F. oxysporum. Tomato PGIP showed different inhibitory activity on PG extracted from different phytopathogenic isolates of F. oxysporum.
Key words: Polygalacturonase inhibiting protein (PGIP), Fusarium oxysporum f.sp lycopersici, polygalacturonase (PG).