ISSN 2756-3324
African Journal of Medicine and Surgery ISSN 2756-3324 Vol. 11 (6), pp. 001-007, June, 2024. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full Length Research Paper
Simulation of Living Intra-Epidermal Micro Patterns to Facilitate Guided Tissue Regeneration of the Human Skin
Denis E. Solomon
96 Standishgate, Wigan WN1 1XA, England. E-mail: [email protected].
Accepted February 26, 2024
Abstract
The methodology for isolating living intra-epidermal micro patterns using a tissue culture technique is detailed. The disassembly of the stripped epidermis (after Dispase digestion at 37°C) into epidermal brown rosettes (observed under a phase contrast microscope) forms intra-epidermal micro patterns. The stepwise reasoning for identifying epidermal basal layer rosettes and the distinction between attached upright and inverted brown rosettes spreading their epidermal cell contents onto a prepared extracellular matrix is explained. Mimicking the circular shape of brown rosette layers using a microscopic sterile cornstarch granule (appearing donut-shaped under the microscope) as a biodegradable nutritional scaffold is proposed. A lightweight antibiotic ointment combined with the cornstarch granules was utilized, and the significance of this mixture in guided tissue regeneration of the epidermis after Mohs surgery for basal cell carcinomas, as a treatment for second-degree burns, and for potential healing of donor sites after skin biopsies is discussed.
Key words: Natural micro patterns, Mohs surgery, basal layer, tissue repair, epidermal-melanin unit, human epidermis, Cellular Potts Model, microscopic nutritional cell scaffold.