ISSN 2756-3391
African Journal of Parasitology Research ISSN 2756-3391 Vol. 7 (4), pp. 001-003, April, 2020. © International Scholars Journals
Short Communication
Detection of Streptococcus iniae by polymerase chain reaction in rainbow trout (Oncorhynchus mykiss) in west Iran
Firooz Fadaeifard1*, Mahdi raissy1, Hasan Momtaz2 and Reza Zahedi3
1Department of Aquatic Health and Disease, Shahrekord Branch, Islamic Azad University-Shahrekord-Iran.
2Department of Microbiology, Shahrekord Branch, Islamic Azad University-Shahrekord-Iran.
3Graduated of Shahrekord Branch, Islamic Azad University-Shahrekord-Iran.
Accepted 23 January, 2020
Abstract
Streptococcosis is one of the serious diseases in cultured salmonids, specially rainbow trout, Oncorhynchus mykiss. Streptococcus iniae is one of the important pathogens for fish which is also dangerous for human. The aim of this study is to investigate the streptococcosis caused by S. iniae in rainbow trouts farms. For this purpose, we evaluate fishes that showed clinical signs similar to streptococcosis from 10 farms in west of Iran on the subject of S. iniae existence. At first, the specific culture for streptococcus was done and, then, polymerase chain reaction (PCR) for detection of S. iniae was used. Out of 50 evaluated specimens, 13 were suspected to S. iniae after biochemical tests. PCR uses specific primers for S. iniae and 6 samples were revealed positive. The results of this study showed the high existence of S. iniae in this region. PCR is an effective technique for the rapid and specific detection of S. iniae in fish tissues.
Key words: Streptococcus iniae, polymerase chain reaction (PCR), Chaharmahal –va- Bakhtyari Province, Iran.