ISSN 2167-0404
International Journal of Medicine and Medical Sciences ISSN: 2167-0404 Vol. 3 (5), pp. 434-440, May, 2013. © International Scholars Journals
Full Length Research Paper
Evaluation of a malaria rapid diagnostic test among febrile children in Sokoto, Nigeria
*Sani UM, Jiya NM, Ahmed H
Department of Pediatrics, Usmanu Danfodiyo University Teaching Hospital, (uduth) Sokoto, Nigeria.
*Corresponding author. Email: [email protected]
Accepted 01 May, 2013
Abstract
Malaria rapid diagnostic test (RDT) is an antigen capture assay that enables rapid diagnosis of malaria without the need for electricity or highly skilled technicians. Though potentially useful, its adoption needs to be guided by local test sensitivity. This study evaluated the diagnostic performance of a commercially available RDT (Malaria Pf rapid device, Biotec Laboratories Limited, United Kingdom) among 400 febrile children(aged 6months to 12 years) in Sokoto, Nigeria. It was a prospective observational study conducted at the Paediatric Outpatient Department (POPD) of UDUTH, Sokoto between March and October, 2009. Finger prick blood samples were collected from each of the patients (day 0) and immediately tested for falciparum malaria by both Giemsa microscopy and rapid diagnostic test (RDT). Patients with both positive RDT and positive microscopy results on day 0 were retested on day 7 (after antimalarial therapy) by both diagnostic methods. The prevalence of malaria among the study cohort was 40.8% by microscopy and 39.5% by RDT. The RDT had a sensitivity of 90.2% and specificity of 95.4%; with positive and negative predictive values of 93.0% and 93.4% respectively. Test accuracy was 93.3%, whereas reliability was 85.3%. Test sensitivity is reduced by low parasite density (100% at > 1600/µl Vs 69.2% at <800/µl). Of the 69 patients who were retested on day 7 after antimalarial treatment, 18 (26.1%) still had positive RDT test even though negative by microscopy and afebrile at the time of follow up. The diagnostic performance of the RDT in this study was good. Hence, it is recommended as an alternative method for diagnosis of malaria, especially when microscopy is not feasible.
Key words: Malaria, rapid diagnostic test, children, Sokoto, Nigeria.