ISSN 2756-3413
African Journal of Virology Research ISSN 3421-7347 Vol. 13 (4), pp. 001-005, April, 2019. © International Scholars Journals
Full Length Research Paper
Biochemical studies on changes associated with enzymes of glucose metabolism in white spot syndrome virus (WSSV) infected with Penaeus monodon (Fabricius)
Suseela Mathew1*, Ashok Kumar Kesavan Nair 2, Rangasamy Anandan1, Pullanniyil Gopalan Nair Viswanathan Nair1, and Koodenchery Devadasan1
1Biochemistry and Nutrition Division, Central Institute of Fisheries Technology, Matsyapuri (PO), Cochin 682 029, India.
2Quality Assurance and Management Division, Central Institute of Fisheries Technology, Matsyapuri (PO), Cochin 682 029, India.
Accepted 16 January, 2019
Abstract
Tiger prawns ( Penaeus monodon) were infected with white spot virus artificially by intramuscular injection of the virus inoculum. Haemolymph, hepatopancreas and muscle samples from the infected prawns were analyzed for glucose and enzymes viz aldolase, glucose-6-phosphatase, fructose-1,6-diphosphatase and glucose-6-phosphate dehydrogenase in the carbohydrate metabolism. Glucose content reduced to 33% of its original value in 24 h of infection and to 31% in 48 h. Almost 95% loss in activity was observed in the case of fructose 1,6-diphosphatase in hepatopancreas, whereas the reduction in activity in muscle was 67%, after 48 h of infection. Glucose-6-phosphatase showed a reduction of 16 and 13% in hepatopancreas and muscle respectively during 24 h of infection. The activity of glucose-6-phosphate dehydrogenase increased by 300% of original value in hepatopancreas, while in muscle the increase was only 30% of original value during 24 h of infection. No significant change in activity was noted in the case of aldolase. Even at moribund stage, the glycolytic pathway was not affected, as evident from the normal activity of aldolase observed in the present study.
Key words: WSSV, P. monodon, glucose, gluconeogenic enzymes, glycolytic enzymes.