ISSN 2756-3855
International Journal of Urology and Nephrology ISSN 2091-1254 Vol. 8 (3), pp. 001-007, March, 2020. © International Scholars Journals
Full Length Research Paper
Synergistic effects of squalene and polyunsaturated fatty acid concentrate on lipid peroxidation and antioxidant status in isoprenaline-induced myocardial infarction in rats
N. Dhandapani1,2, B. Ganesan1,3, R. Anandan 3*, R. Jeyakumar1, D. Rajaprabhu1, R. Anbin Ezhilan1
1Department of Biochemistry, Vinayaka Mission’s Research Foundation (Deemed University), Ariyanoor, Salem-636308, India.
2Department of Pharmaceutical Chemistry, R.V.S College of Pharmaceutical Sciences, Sulur, Coimbatore-641402, India.
3Biochemistry and Nutrition Division, Central Institute of Fisheries Technology, Matsyapuri (PO), Cochin-682029, India.
Accepted 12 January, 2020
Abstract
We have studied the synergistic effects of squalene and polyunsaturated fatty acids (PUFA concentrate) on isoprenaline-induced infarction in rats with respect to changes in the levels of plasma diagnostic marker enzymes and myocardial antioxidant defense system. Intraperitoneal injection of isoprenaline caused a significant elevation in the levels of diagnostic marker enzymes; alanine aminotranferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) in plasma of experimental rats. There was a significant rise in the level of lipid peroxidation with concomitant decline in the level of reduced glutathione (GSH) and in the activities of glutathione-dependent antioxidant enzymes; glutathione peroxidase (GPX) and glutathione-S-transferase (GST), and antiperoxidative enzymes; superoxide dismutase (SOD) and catalase (CAT) in heart tissue. Combined supplementation of squalene and PUFA concentrate significantly prevented the isoprenaline-induced elevations in the levels of diagnostic marker enzymes in plasma of experimental groups of rats. A tendency to counteract the isoprenaline induced lipid peroxidation was also noticed. Their combined administration maintained the level of GSH and the activities of glutathione-dependent antioxidant enzymes and antiperoxidative enzymes at near normalcy. The results of the present study indicated that the combined administration of squalene and PUFA concentrate exerted significantly better cardio-protection against isoprenaline-intoxication as compared to that of per second supplementation.
Key words: Squalene, PUFA, isoprenaline, myocardial infarction, lipid peroxidation, antioxidant defense system.