African Journal of Chemistry

Full Length Research Paper

Larvicidal activity of extracellular secondary metabolites from a Stereum species Hill ex Pers. (JO5289) against the dengue fever mosquito, Aedes aegypti (Linn) (Diptera: Culicidae)

Dennis Kipngeno Chirchir, Russell Ben Omondi Ouma, Peter Kiplagat Cheplogoi and Josiah

Ouma Omolo*

Department of Chemistry, Egerton University, P.O. Box 536 20115-Egerton, Kenya.

*Corresponding author. E-mail: [email protected], [email protected]. Tel: +254 722 488821. Fax: +254 51 2111112.

Accepted 30 September, 2013

Abstract

The main objective of this investigation was to find mosquito larvicidal secondary metabolites from a basidiomycete – Stereum species (JO5289) – against Aedes aegypti. The Stereum species (JO5289) was collected in July 2005 from undisturbed habitat in Londiani forest in Rift Valley province, Kenya. Extracellular crude extracts from Stereum species (JO5289) produced strong activity against A. aegypti larvae. Purification of the crude extracts targeting larvicidal activity using chromatography gave three active compounds namely; tyrosol, 3-methoxy-5-methyl-1,2-benzenediol and 2-hydroxy-4-(4-hydroxy-chroman-7yl) but-3-enal. The chemical structures of the compounds were determined using the nuclear magnetic resonance (NMR) spectral data and comparison with literature values. When tested for larvicidal activity, the LC₅₀ for the three compounds were 26.7, 17.3 and 14.5 ppm, respectively, while LC₉₀ were 85.3, 83.5 and 82.9 ppm, respectively, after 24 h of exposure. These compounds have been produced from cultures of a Stereum species and reported to have mosquito larvicidal activity for the first time.

Key words: Basidiomycete, Stereum species, Aedes aegypti, extracellular metabolites and larvicidal.