ISSN 2756-3294
African Journal of Botany ISSN: 3519-3824 Vol. 8 (2), pp. 001-004, February, 2020. © International Scholars Journals
Full Length Research Paper
The uncertainty of assessing aflatoxin B1-producing ability using aflR gene in Aspergillus species
Bao-Sheng Wang1, Qian Zhao1* and You-Zhi Wang2,3
1State Key Laboratory of Agro biotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
2CGMCC, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
3Tibet Institute of Plateau Biology, Lhasa 850008, China.
Accepted 19 September, 2019
Abstract
Aspergillus section Flavi consists of many species which are very similar in morphology and very important in human health and food industry. Aspergillus flavus, Aspergillus parasiticus, and Aspergillus toxicarius are notorious aflatoxigenic species. Aspergillus oryzae and Aspergillus sojae are non-aflatoxigenic species and widely used for Oriental fermented food. The presence of the aflR gene and aflatoxin B1 (AFB1) production ability was assessed by PCR detection and HPLC analysis, respectively. The investigation of AFB1 and amplification of the aflR gene in thirty five authentic strains of Aspergillus section Flavi showed that (1) all the AFB1-producing strains were detected to possess the aflR gene; (2) some non AFB1-producing Aspergillus section Flavi strains are constitutional, which did not tested to possess the aflR gene; (3) some strains used in Oriental fermentation have the aflR gene but they are non AFB1-producing aspergilli. The conclusion is that it is uncertain to judge the aflatoxigenicity of an Aspergillus strain using the aflR gene as an indicator.
Key words: Aspergillus, aflatoxin, alfR gene, HPLC, PCR.