ISSN 2756-3413
African Journal of Virology Research ISSN 3421-7347 Vol. 8 (1), pp. 001-008, January, 2014. © International Scholars Journals
Full Length Research Paper
cDNA cloning and expression of anthocyanin biosynthetic genes in wild potato (Solanum pinnatisectum)
Qi-Neng Lu1,2, Qing Yang1*
1Department of Biochemistry and Molecular Biology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
2Department of Agronomy, Yichun University, Yichun 336000, China.
Accepted 13 October, 2013
Abstract
The purple anthocyanins in potato tuber skin and flesh are primarily derived from petunidin. cDNA clones encoding the enzymes involved in anthocyanin biosynthesis, chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), dihydroflavonol 4-reductase (DRF), and UDP-glucose: flavonoid 3-0-glucosyltransferase (3GT), were isolated from Solanum pinnatisectum by RT-PCR with degenerated primers. Sequence comparison showed that they share 76-96% identities with each corresponding solanaceous gene reported previously. Each gene is a member of a multigene family. The spatial expression analysis indicated that these genes were preferentially expressed in flowers, stolons and terminal buds, and their transcripts could not detected in roots except 3GT. All the genes were induced expression in tuber skins by white light, and followed by anthocyanin accumulation. This is the first report cloning and expression of anthocyanin biosynthetic genes in wild potato (Solanum pinnatisectum).
Key words: Anthocyanin, expression, gene cloning, wild potato species.