ISSN 2375-1061
African Journal of Agricultural Marketing ISSN 2375-1061 Vol. 11 (1), pp. 001-006, January, 2023. © International Scholars Journals
Full Length Research Paper
Genetic diversity analysis of Iranian citrus varieties using micro satellite (SSR) based markers
M. Jannati1, R. Fotouhi1, A. Pourjan Abad2* and Zivar Salehi3
1Department of Horticulture, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran.
2Agric-Natural Research Center of Yazd, Genomics Laboratory, Yazd, Iran.
3Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran.
Accepted 8 June, 2022
Abstract
Fifteen SSR Primer Pairs were used to estimate the level of polymorphism among 23 Citrus genotypes and four natural hybrids or bud mutation was selected from Kotra Germplasm Bank (IRAN) . All fifteen loci assayed in citrus plant possessed a high level of polymorphism, with the number of alleles per locus ranging from 4 in TAA41 to 12 at CAT01, ATC09, AG14 ( an average, 8.27 alleles were detected per locus ). Cluster analysis with SSR markers resulted in 2 cluster groups: Group A: Yuzo and Poncirus. Group B: There are three separate subgroups within Group B; (i) genus Fortunella sp (ii) Mandarin subgroup: Citrus reticulate (Citrus clemantin), Citrus sinensis (Pineapple, Washington Navel), Natural types (Siahvaraz, Shalmahaleh, Moallemkoh and Kotra 4 hybrids) and (iii) Citrus Limon (Amol lemon - pear, Eureka, Rough Lemon), Citrus aurantifolia, Citrus aurantium, Citrus medica and Citrus grandis. Microsatellite analysis clustered citron and sour orange cv cluster but these taxa were quiet distant from Fortunella SP.
Key words: Citrus, microsatellite, phylogeny, polymorphism, germplasm bank, genetic diversity.