ISSN 2756-3855
International Journal of Urology and Nephrology ISSN 2091-1254 Vol. 7 (3), pp. 001-005, March, 2019. © International Scholars Journals
Full Length Research Paper
Amelioration of carbon tetrachloride induced oxidative stress in kidney and lung tissues by ethanolic rhizome extract of Podophyllum hexandrum in Wistar rats
Showkat Ahmad Ganie, Ehtishamul Haq, Akbar Masood and Mohmmad Afzal Zargar*
Department of Biochemistry, University of Kashmir, Srinagar, 190006, India.
Accepted 25 July, 2018
Abstract
Antioxidant activity of 70% ethanolic extract of rhizome of Podophyllum hexandrum (Indian Mayapple) was investigated in rats by inducing kidney and lung injury with CCl4. 70% ethanolic extract exhibited significant antioxidant activity in the tissues by showing increased levels of proteins, reduced glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), and glutathione-S-transferase (GST) as well as decreased levels of thiobarbituric acid reactive substances (TBARS). The 70% ethanolic extract at a dose level of 20, 30 and 50 mg/kg-day, showed a dose dependent restorative activity against CCl4-induced kidney and lung tissue damage. In our previous study 70% ethanolic extract of P. hexandrum had displayed a potent free radical scavenging activity under in vitro conditions. In the present study, the 70% ethanolic extract of P. hexandrum was effective in restoring CCl4-intoxicated kidney and lung tissue enzymes towards normalcy in combating oxidative stress in vivo. Thus, the extract has antioxidative activity.
Key words: Antioxidants, carbon tetrachloride, Podophyllum hexandrum, GSH, catalase.