ISSN 2756-3391
African Journal of Parasitology Research ISSN 2756-3391 Vol. 11 (1), pp. 001-009, January, 2023. © International Scholars Journals
Full Length Research Paper
Genetic Characterization of Campylobacter jejuni Isolated from Boiler Flocks
H. Abo Al-Yazeed1, Amani M. Marwan1, Attia, A. M.2, A. Samir1, Ammar, A. M3
1Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt.
2Department of Bacteriology, Animal Health Research Institute, Dokki, Giza.
3Microbiology Department, Faculty of Veterinary Medicine, Zagazig University, Egypt.
Accepted 05 October, 2022
Abstract
Campylobacter is a worldwide infection which has been estimated it as the most significant economic burden by EFSA and ECDC in 2016. It caused mainly by either Campylobacter jejuni or Campylobacter coli. Poultry are a natural reservoir for Campylobacter species, although it is insignificant for poultry health and is a leading cause of gastroenteritis for humans in developed and developing countries. The disease is endemic in Egypt and is a major cause for diarrhea in children. Good understanding of epidemiology and surveillance of Campylobacter will help in elimination and prevention of it among animals and humans. Many molecular typing techniques used to track the source of infection and reduce Campylobacter infection rate. To achieve that, 290 samples were collected from broiler flocks and slaughter market from Cairo governorate, Egypt. Bacteriological and molecular identification have been implemented based on genus and species level for C. jejuni then phylogenetic tree analysis of flaA gene to correlate the genetic relatedness and emphasize that chicken is the major source of infection.
Keywords: Campylobacter jejuni, genotyping, flaA gene, short variable region, chicken, Human.