ISSN 2326-7275
International Journal of Anatomy and Physiology ISSN: 2326-7275 Vol. 7 (2), pp. 001-009, February, 2018. © International Scholars Journals
Full Length Research Paper
Preventive effect of Rhododendron arboreum on cardiac markers, lipid peroxides and antioxidants in normal and isoproterenol-induced myocardial necrosis in rats
Manjunatha P. Mudagal*, Sandip Karia and Divakar Goli
Department of Pharmacology, Acharya and B. M. Reddy College of Pharmacy, Soladevanahalli, Chikkabanavara Post, Bangalore- 560090, Karnataka, India.
Accepted 21 April, 2017
Abstract
This study was designed to investigate the preventive effect of ethanolic extract of Rhododendron arboretum (ERA) against isoproterenol-induced myocardial ischemia in rat myocardium. Wister rats were pretreated with ERA (100, 250, 500 mg kg-1) orally for 42 days and then intoxicated with isoproterenol (ISO, 150mg/kg body weight, s.c. for 2 days). MI induced in rats by subcutaneous injection of ISO-(150 mg/kg body weight) at interval of 24 h for 2 days showed significant elevation in serum cardiac marker enzymes like lactate dehydrogenase (LDH), aspartate transaminase (AST) and alanine transaminase (ALT) . Lipid peroxidative product like lipid hydroperoxidase was elevated in tissue and serum, decline in enzymatic antioxidant status (superoxide dismutase, catalase, glutathione peroxidase) and non-enzymatic reduced glutathione antioxidants. Pretreatment with ERA to ISO-treated rats caused a significant protective effect. Administration of ERA in normal rats did not have any significant effect on cardiac markers, lipid peroxidation and antioxidant levels. The results of our study show that ERA possesses protective effect result from suppression of oxidative stress in ISO-induced myocardial infracted rats. Histopathological examination further confirmed the cardioprotective effect of ERA against ischemic insult.
Key words: Myocardial infarction, isoproterenol, antioxidants, Rhododendron arboreum, oxidative stress.