ISSN 2756-388X
International Journal of Plant and Animal Sciences ISSN 2167-0366 Vol. 5 (2), pp. 001-004, February, 2017. © International Scholars Journals
Full Length Research Paper
DGGE-RAPD analysis as a useful tool for cultivar identification
A. Bahieldin1,2*, I.A. Ahmed3, Gh.A. Gad El-Karim1, H.F. Eissa1, H.T. Mahfouz1 and O.M. Saleh4
1Agricultural Genetic Engineering Research Institute (AGERI), ARC, Giza, Egypt.
2Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt.
3Barley Department, Field Crops Research Institute, ARC, Giza, Egypt.
4National Center of Radiation Research and Technology, Egypt.
Accepted 16 November, 2017
Abstract
Denaturing gradient gel electrophoresis- random amplified polymorphic DNA (DGGE-RAPD) was used to overcome the main drawbacks of RAPD (i.e., the low levels of reproducibility and polymorphism). As a model, six barley cultivars of known origin were tested for RAPD markers using DGGE methodology with 29 arbitrary primers. Among a total of 418 bands observed, as high as 99 were polymorphic. Comparison between agarose-RAPD and DGGE-RAPD revealed that the latter was highly reproducible and gave higher level of polymorphism and consequently more markers. The relationships among barley cultivars derived from this study based on DGGE- RAPD are consistent with the known lineage of these cultivars. In conclusion, we recommend the use of DGGE-RAPD as an alternative tool to the more costly DNA-based analysis in cultivar identification in laboratories with limited funds.
Key words: Denaturing gradient gel electrophoresis-random amplified polymorphic DNA, artificial heteroduplex, dendogram, lineage.