International Journal of Urology and Nephrology ISSN 2091-1254 Vol. 4 (1), pp. 101-109, January, 2016. © International Scholars Journals
Full Length Research Paper
Analysis of proteomic and acute kidney allograft rejection in rat
Wu Yi1, Li Peng2 and Wang Xueqian3
1Department of Urology, the First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China.
2Department of Emergency, the First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China.
3Department of Laboratory Medicine, the First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China.
*Corresponding author. E-mail: [email protected].
Received 12 October, 2015; Revised 04 January, 2016; Accepted 10 January 2016 and Published 22 January, 2016
Abstract
Kidney transplantation to treat end-stage renal disease has evolved rapidly from the first successful transplantations to the current widespread use of grafts from both cadaveric and living donors. But acute rejection is still a strong risk factor for chronic rejection in recipients of renal grafts. To investigate possible mechanisms, we describe a comparison between different proteins expression profile of acute rejection and the controls. Through two-dimensional difference gel electrophoresis, mass spectral techniques microarray analysis and quantitative real-time RT-PCR confirmation, marked change in expression level of 37 protein spots was observed in allograft rejection group. Among them, expression levels of 24 protein spots were up-regulated, while expression levels of 13 protein spots were down-regulated. Among 37 protein spots, 29 obtained satisfactory peptide mass fingerprinting. We identified 6 proteins in acute rejection after renal transplantation. Then, we investigated PDIA3 levels in serum and transplanted kidney, PDIA3 mRNA and protein expression level. Our data thus indicate that PDIA3 might be potentially involved into the occurrence and development of acute rejection response in renal transplantation.
Key words: PDIA3, mass spectrum, acute rejection, renal transplantation.