ISSN 2375-1231
African Journal of Crop Science ISSN: 2375-1231 Vol. 12 (6), pp. 001-009, June, 2024. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full Length Research Paper
Pathogenicity of satellite associated with cassava Begomovirus in Côte D’Ivoire
K. Kouamé Daniel, T. Marie Noël Yeyeh, and K. Yadom Yao François Régis
Natural Sciences Department, Laboratory of Plant health, University Nangui ABROGOUA, 02 BP 801 Abidjan 02, Côte d’Ivoire.
Received 3 April, 2024; Accepted 8 May, 2024 and Published 11 June, 2024
Abstract
Africa represents more than 50% of global cassava production. A study was carried out in Côte d'Ivoire in Abidjan, Abengourou, Grand-Lahou and Toumodi. In cassava and Chromolaena odorata, similar symptoms of virus disease were observed in the fields. Mosaic deformations and discolorations were observed in infected leaves with medium to very high severity. These collected leaves were subjected to total DNA extraction and conventional PCR targeting the Begomoviruses gene. Multiplex PCR was performed with specific primer pairs CMBrep/F, ACMVrep/R and EACMVrep/R for the detection of CMV and ACMV in cassava and C. odorata leaves. Additional PCR was performed with the specific primer pair JPS001/JPS002 for detection of ACMV, followed by PCR with primers JPS001/JPS003 for Begomovirus satellite detecting in both leaf samples. PCR confirmed the presence of ACMV, EACMV, Sat II and Sat III satellites, associated with cassava Begomovirus DNA in infected leaves with the highest severity for both cassava and C.odorata.C. odorata could ensure the rapid spread of the very serious cassava virus in the fields of farmers in Côte d'Ivoire. It would be important to consider C. odorata in the management of cassava viral diseases.
Key words: Begomovirus, Cassava, Chromolaena odorata, Satellites, Pathogenicity.