ISSN 2167-0447
International Journal of Agricultural Sciences ISSN: 2167-0447 Vol. 3 (10), pp. 040-045, December, 2013. © International Scholars Journals
Full length Research Paper
Callus induction and regeneration of potato from shoot tip culture
Umme Zohora Laboney1, Gokul Chandra Biswas2*, Mohammad Abdullah-Al-Shoeb3 and Md. Abunasar Miah2
1Department of Biotechnology, Faculty of Agriculture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
2Department of Genetic Engineering and Biotechnology, School of Life Sciences, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh.
3Department of Biochemistry and Molecular Biology, School of Life Sciences, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh.
*Corresponding Author. Email: [email protected]
Accepted 16 September, 2013
Abstract
Two laboratory experiments were performed with potato cv. Granula to investigate callus induction ability and regeneration capability from shoot explants and to study the influence of BAP, IAA and GA3 on plantlet regeneration from callus. In the first experiment, MS medium with 2.0 mg/l 2,4-D were used to identify the highest callus formation (95.00%) within a minimum number of days (10.00), number of calli /culture (4.75) and weight of callus (0.074 g). However, the second experiment was done for both of shoot and root formation. In case of shoot regeneration, combination of 1.0 mg/l BAP and 0.5 mg/l GA3 showed the highest number of shoots per explants (4.67), shoot initiation (93.33%) at required days (15.00), number of leaves per plantlet (3.67) and longest shoot (5.33 cm). On the other hand, the combination of 1.0 mg/l IAA and 1.0 mg/l GA3 showed the highest number of roots per shoot (3.33) within a minimum number of days (12.33) and longest root (6.33 and 7.33 cm) after 20 and 28 days, respectively. Therefore, it was concluded that the hormonal concentration to effect the callus formation and regeneration. Further attempts have been used for production of disease free potato variety from this study.
Keywords: Callus, culture medium, in vitro, regeneration, shoot tip.