ISSN 2736-173X
African Journal of Malaria and Tropical Diseases ISSN 2736-173X Vol. 12 (6), pp. 001-005, June, 2024. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full Length Research Paper
Molecular Cloning and Characterization of the Tpn I Gene in Bombyx mori
Zhao Yuan1, 2, Yao Qin1, Tang Xudong1, Wang qiang1, Yin huijuan1, Hu Zhigang1, Lu Jian1 and Chen Keping1*
1Institute of Life Sciences, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, P. R. China.
2 Sericultural Research Institute, Chinese Academy of Agricultural Sciences, 212018, China.
Accepted 16 February, 2024
The troponin complex is composed of three subunits, Troponin C (the calcium sensor component) and Troponin T and I (structural proteins). Tpn C is encoded by multiple genes in insects, while the Tpn T and Tpn I proteins are encoded by single genes. Tpn I binds to actin and Tpn T binds to tropomyosin. We cloned and sequenced the Tpn I (AY873787) gene from Bombyx mori that encodes 225 amino acids and contains a conserved motif seen in Drosophila virilis and Anopheles gambiae. Bioinformatic analysis suggests that its deduced amino sequence shares 81.3 and 78.7% homology with the Tpn I genes of A. gambiae and D. virili, respectively.
Key words: Tpn I, Bombyx mori, troponin, homology.