ISSN 2326-7267
International Journal of Pharmacy and Pharmacology ISSN 2326-7267 Vol. 14 (3), pp. 001-007, March, 2025. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full Length Research Paper
RP-HPLC Assay for Levofloxacin in Rat Plasma and Saliva: Development, Validation, and Pharmacokinetic Applications
Naheed W. Sheikh1*, Alok S. Tripathi1, V. Chitra1, Avijit Choudhury1 and Anil P. Dewani2
1Department of Pharmacology, SRM College of Pharmacy, SRM University, Kattankulathur- 603203, Tamil Nadu, India. 2Department of Pharmaceutical Analysis, P.W. College of Pharmacy, Dhamangaon Road, Yavatmal, 445001, Maharashtra, India.
Accepted 19 September, 2024
Abstract
A new, simple, specific, accurate and precise reversed phase-high performance liquid chromatography (RP-HPLC) method was developed for the determination of levofloxacin in rat plasma and saliva was developed. An HPLC system based on a Phenomenex Luna C18 Column (250 × 4.6 mm) and a UV detector ( = 296 nm) were used. A mixture of Acetonitrile: water (80:20 v/v) adjusted to pH 3.5 by orthophosphoric acid at a flow rate of 1.4 ml/min was used as mobile phase. The proteins were precipitated with methanol. The average recovery was 94.79 and 92.66%, respectively in plasma and saliva. The detection limit for levofloxacin in plasma and saliva was 1 µg/ml. The calibration curve was linear over the concentration range 1 to 16 mg/ml for plasma and saliva. The inter-day and intra-day assay coefficients of variation were found to be less than 5%. The present validated method was successfully used for pharmacokinetic studies of levofloxacin in plasma and saliva.
Key words: High-performance liquid chromatography (HPLC), levofloxacin, plasma, saliva, pharmacokinetics.