African Journal of Parasitology Research

ISSN 2756-3391

African Journal of Parasitology Research  ISSN 2343-6549 Vol. 4 (7), pp. 235-243, September, 2017. © International Scholars Journals

Full Length Research Paper

Associations between IgG antibody responses to multiple Plasmodium falciparum antigens and treatment outcomes with ACTs in Man, Cote d’Ivoire

Yao SS1, Offianan AT1, Tiacoh NL1, Ako AAB1, Koffi D1, Kouame E1, Tuo K1, Beourou S1, Djaman J2

1Malariology Department, Institut Pasteur Côte d’Ivoire.

2Biochemestry Department Institut Pasteur/UFR Biosciences Félix Houphouët Boigny University.

Corresponding Author E-mail: [email protected]

Tel. (00225) 40 33 57 83/ (00225) 49 45 91 03

Accepted 18 September, 2017

Abstract

Successful antimalarial drugs treatment might be due to intrinsic susceptibility of P. falciparum and host’s factors. In this study we assessed relationship between host IgG antibody responses and treatment outcome with Artesunate+Amodiaquine (AS+AQ) and Artemether+Lumefantrine (AL) in high transmission setting of Cote d’Ivoire. The study was done as part of a clinical drug-efficacy trial of AS+AQ and AL conducted at Man in western Côte d’Ivoire. Serum samples from the time of malaria diagnosis and 7 days later were analyzed for total IgG against schizonte extract (07/03) and 5 specific-P. falciparum Ags using a quantitative indirect ELISA. IgG antibody responses were related to treatment outcome. A total of 56 and 55 serum samples in AL and AS + AQ group respectively were available for antibody analysis, on day 0 and day 7.  The levels IgG to CSP, GLURP and SALSA increased significantly with age (p<0.05). Antibody responses against all antigens decreased significantly, except SALSA and sch07/03 in day 7 after treatment, with the greatest decreasing seen in IgG response to GLURP (p= 0.0013). Adequate Clinical and Parasitological Response PCR corrected (ACPR) was of 98.3% (AS +AQ) and 100% (AL). Prevalence and levels of anti-Glutamate-rich Protein (GLURP) and anti-circumsporozoite (CSP)-specific IgG antibodies were significantly higher (P < 0.001) in Patients with ACPR in AS+AQ and AL group respectively.  GLURP and CSP IgG antibody responses in Man region may contribute to malaria treatment success and support hypothesis that acquired immunity enhances clinical efficacy of antimalarial dugs.

Keywords: IgG Antibody responses, treatment outcome, ACT, Man, Côte d’ivoire