ISSN 2167-0447
International Journal of Agricultural Sciences ISSN 2167-0447 Vol. 5 (3), pp. 435-45, May, 2014. © International Scholars Journals
Full Length Research Paper
Investigating the effect of storage time and growth regulator furolan on the contents of protein fraction composition and amino acids in winter wheat grain
*Essien Okon1, Hannah Etta1 and Natali Nenco2
1Department of Biological Science, Cross River University of Technology, Calabar, Nigeria.
2Department of Food Engineering and Advanced Technology, Kuban State University of Technology, Krasnodar, Russia.
Abstract
This investigation was carried out to study the effect of storage time and growth regulator, Furolan (the extrinsic factors) on the contents of protein fraction composition and amino acids (the intrinsic factors) of three varieties of winter wheat grains namely Batko, Diya and Krasnodar 99. The determination of mass fraction of amino acids and fractional composition of protein complex was respectively carried out by capillary electrophoresis and Osborne methods. During harvest, Furolan-treated varieties of Diya (42.9%) and Batko (41.7%) were found to differ from Furolan-treated Krasnodar 99 (48.3%) variety by their low content of albumin and globulin and the high content of alcoholic-and alkaline- soluble fractions of protein (protein gluten) due to the genetic potential of these varieties. During storage there was a decrease in the albumin and globulin contents and an increase in the protein gluten content of Krasnodar 99 variety in the control. It was observed that, during 12 months of storage the contents of cyclic amino acids in all the varieties studied decreased. Similarly, there was a decrease in monoaminomonocarboxylic amino acids (glysine, alanine, serine, threonine, valine, leucine) content of the average-ripening (Diya and Krasnodar 99) varieties.
Key words: Furolan, storage, winter wheat, protein, amino acids, gluten.