International Journal of Medicinal Plants Research

ISSN 2169-303X

International Journal of Medicinal Plants Research ISSN 2169-303X Vol. 9 (9), pp. 001-007, September, 2020. © International Scholars Journals

Full Length Research Paper

Phenolics from Spirulina maxima: Over-production and in vitro protective effect of its phenolics on CClinduced hepatotoxicity

Hanaa H. Abd El-Baky1*, Farouk K. El Baz1 and Gamal S. El-Baroty2

1Plant Biochemistry Department, National Research Centre, Dokki, Cairo, Egypt.

2Department of Biochemistry, Faculty of Agriculture, Cairo University, Egypt.

Accepted 14 July, 2020

Abstract

This study illustrates the process of enhancing phenolics synthesis in Spirulina maxima grown in Zarrouk’s medium supplemented with sodium nitrate (NaNO3) and/or phenylalanine (L-PA); attaining highest production were obtained in medium containing 3.77 gL-1 NaNO3 and 100 mgL -1 L-PA. HPLC profile showed the presence of phenolic acids and flavonoids predominantly with gallate, chlorogenate, cinnamate, pinostrobate and p-OH-benzoates. The protective action of Spirulina phenolic compounds (SPC) against CCl4-induced in vitro, hepato-toxicity symptoms like microsomal lipid peroxidation and hydroxyl radical formation were studied. SPC exhibited antioxidant effects on DPPH radical scavenging with IC50 values ranging from 23.22 to 35.62 µg.mL-1 and inhibited CCl4-induced lipid peroxidation in hepato-microsoms model, in dose-depended manner. Their protective potential was comparable to that of standard phenolic antioxidants such as BHT, BHA and –tocopherol (IC50 values ranged from 13.22 to 23.62 µg.mL-1).

Key words: Spirulina maxima, antioxidant activity, carbon tetrachloride, hepatoprotective effects.