African Journal of Pig Farming

ISSN 2375-0731

African Journal of Pig Farming ISSN 2375-0731 Vol. 5 (12), pp. 001-006, December, 2017. © International Scholars Journals

Full Length Research Paper

Comparative proteomic analysis of the effects of dietary mugwort (Artemisia iwayomogi Kitamura) on the pig Longissimus dorsi muscle

Byung Uk Kim1, Mi Ae Jeong1, Yeon Sun Ryu2, Hwa Chun Park 3, Jong Hyun Jung3, Sam Woong Kim4, Chul Wook Kim1, Young Min Song1, Ki Hwa Chung1, Il Suk Kim1, Sang Keun Jin1, Sang Suk Lee5, In Soon Choi6 and Kwang Keun Cho1*

1Department of Animal Resources Technology, Gyeongnam National University of Science and Technology, 150 Chilam-dong, Jinju, Gyeongnam, 660-758, Republic of Korea.

2Department of Animal Health Management, Woosuk University, 443 Samnye-eup, Wanju-gun, Jeonbuk, 565-701, Republic of Korea.

3Dasan Genetics, Gasan-ri, Unbong-ub, Namwon-city Jeonbuk, Republic of Korea.

4Swine Science and Technology Center, Gyeongnam National University of Science and Technology, 150 Chilam-dong, Jinju, Gyeongnam, 660-758, Republic of Korea.

5Department of Animal Science and Technology, Sunchon National University, Suncheon 540-742, Republic of Korea.

6Department of Biological Sciences, Silla University, Busan, 617-736, Republic of Korea. 

Accepted 03 August, 2017

Abstract

Proteomic analysis by 2-dimensional electrophoresis (2-DE) of the longissimus dorsi muscle in finishing pigs (LY × D) grown on a diet supplemented with mugwort powder found roughly 300 spots on a polyacrylamide gel image. Among them, the expression levels of 12 spots were higher than those of the control group. From the results of peptide mass fingerprinting (PMF) analysis, nine proteins were characterized: myosin light chain 2V, elF-5A, myosin light chain 1, F1-ATPase chain D, peroxiredoxin-2, Kelch-related protein 1, SLA-7, glycine amidinotransferase, and Tpi 1. The other three spots with increased expression were identified by chemically assisted fragmentation-matrix-assisted laser desorption/ionization (CAF-MALDI) sequencing as serum albumin precursor and two different types of myoglobin. Taken together, these results indicate that mugwort powder has the potential to improve the quality of pork meat.

Key words: 2-DE, longissimus dorsi muscle, meat quality, mugwort, pig, proteome.

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In Soon Choi and Kwang Keu on Google Scholar In Soon Choi and Kwang Keu on Pubmed Young Min Song on Google Scholar Young Min Song on Pubmed Yeon Sun Ryu on Google Scholar Yeon Sun Ryu on Pubmed Sang Keun Jin on Google Scholar Sang Keun Jin on Pubmed Il Suk Kim on Google Scholar Il Suk Kim on Pubmed Chul Wook Kim on Google Scholar Chul Wook Kim on Pubmed Sang Suk Lee on Google Scholar Sang Suk Lee on Pubmed Ki Hwa Chung on Google Scholar Ki Hwa Chung on Pubmed Byung Uk Kim on Google Scholar Byung Uk Kim on Pubmed Hwa Chun Park on Google Scholar Hwa Chun Park on Pubmed Jong Hyun Jung on Google Scholar Jong Hyun Jung on Pubmed Mi Ae Jeong1 on Google Scholar Mi Ae Jeong1 on Pubmed Sam Woong Kim on Google Scholar Sam Woong Kim on Pubmed