ISSN 2375-0715
African Journal of Fisheries Science ISSN 2375-0715 Vol. 7 (2), pp. 001-004, February, 2019. © International Scholars Journals
Full Length Research Paper
Reverse transcriptase polymerase chain reaction is a sensitive diagnostic tool for detection of nodavirus infection in Macrobrachium rosenbergii
D. P. Behera*, K. Devdas and L. Nayak
Department of Marine Sciences, Berahmpur University, Bhanja Bihar, Berhampur, Orissa-760007,India.
Accepted 13 November, 2018
Abstract
Macrobrachium rosenbergii is the most important cultured Palaemonid in the world. It is farmed on a large scale in many countries including India, once upon a time this species was considered as disease free animal but towards later part of 2001, the hatchery and nursery pond of these animals suffered a massive loss due to a new viral disease named white tail disease. Till date different diagnosis has been established for detection of this disease but reverse transcriptase polymerase chain reaction (RT-PCR) techniques is the most sensitive diagnostic tool among those present available for detection of MrNv (Macrobrachium rosenbergii nodavirus). The designed primer fair (1F/1R) in 1st step PCR was carried out using 1 µl e DNA like wise in 2nd step RT-PCR product the nested primer 2nd 1 µl of 1st RT-PCR product were utilized. At the end of 8 µl of each step PCR product was analyzed by electrophoresis on 1% agarose gel along with sample. The confirm positive and negative sample were run in the gel, the gel electrophoresis of RT-PCR product positive control (Lane-3) is expected amplified product of about 489 bp size of noda virus presence in sample.
Key words: Reverse transcriptase, diagnostic tool, nodavirus, Macrobrachium.