ISSN 2326-7283
African Journal of Internal Medicine ISSN 2326-7283 Vol. 8 (3), pp. 001-006, March, 2020. International Scholars Journals
Full Length Research paper
Experimental chronic inflammation induced in mice periodontium by Actinomyces israelii entrapped in alginate gel
Vagner Rodrigues Santos 1*, João Luis Duval Cândido da Silva1, Sérgio Monteiro Lima Jr1, Felipe Alcântara Braga1, Mariana Passos De Luca1, Vladimir Reimar A. Souza Noronha1, Evandro Guimarães Aguiar1 and Márcio M. Mattos2
1Laboratory of Microbiology and Biomaterials , Facult of Dentistry , Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.
2Laboratory of Pharmacology, Facult of Pharmacy, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.
Accepted 17 September, 2019
Abstract
Experimental chronic inflammation in periodontal actinomycosis was studied with INCQS standard Actinomyces israelii infected mandibula in Swiss/NIH mice (n = 45), male and female with 21 days of age. Tissue responses of the experimentally induced chronic inflammation were investigated in mice by clinical and light microscopy. After anesthetization, the animals were inoculated in paraperiosteal periodontium anterior mandibula with 108 UFC/mL A. israelii (n = 45) associated with sodium alginate gel particles. The animals had been evaluated clinically and microscopically at 1, 3, 7, 15, 21, 30 and 45 days after inoculation. Chronic inflammation was induced in all the mice when compared to the control animals. As such, both groups had normal general health throughout the study. In the control mice (n = 10), no lesions were noted. However, the clinical and histopathology evolutions of actinomycosis lesions had been observed and are discussed in this work.
Key words: Actinomycis israelii, chronic infection, alginate gel, experimental actinomycosis, periodontium, mice.