ISSN 2756-3391
African Journal of Parasitology Research ISSN 2756-3391 Vol. 7 (8), pp. 001-007, August, 2020. © International Scholars Journals
Full Length Research Paper
The in vitro antioxidant properties of Bacillus simplex XJ-25 isolated from sand biological soil crusts
Z. R. Wang1,2, J. P. Sheng1, X. L. Tian2, T. T. Wu2, W. Z. Liu2 and L. Shen1*
1College of Food Science and Nutritional Engineerin, China Agricultural University, Beijing100083, China.
1School of Food Science, Henan Institute of Science and Technology, Xinxiang 453003, China.
Accepted 05 October, 2019
Abstract
XJ-25, a strain with strong antioxidant activity was isolated from sand biological soil crusts in Gurban Tonggut Desert, Xinjiang, China. Strain XJ-25 is closely related to Bacillus simplex through the 16s rDNA sequencing combined with morphological, physiological and biochemical analysis. The medium for optimal antioxidant activity was NB with 1.5 g/L glucose. Based on the kinetic assay, antioxidant activity began at early exponential growth phases; maximum activity was reached at the stationary phase. Scavenging effects on 2, 2-Diphenyl-1-picrylhydrazyl (DPPH), the hydroxyl and superoxide radicals, T-AOC capacity and protection against lipid damage were evaluated. The main antioxidant compounds were in the extracellular secreted supernatant of XJ-25. The active compounds were very stable at a pH range of 2 to 12, and temperature from 40 to 121°C, as well as in some organic solvents. Thin layer chromatography assay by DPPH scavenging assay showed two active spots with a Rf values of 0.35 and 0.47, and both of them were ninhydrin positive.
Key words: Antioxidant activity, Bacillus simplex, TLC, bacteria, sand soil biological crusts.