ISSN 2736-1748
African Journal of AIDS and HIV Research ISSN 2326-2691 Vol. 8 (3), pp. 001-003, March, 2020. © International Scholars Journals
Short Communication
Screening for the presence of Helicobacter pylori in stool of HIV- positive patients
Hossein Samadi Kafil1*, Fatemeh Farahi Jahromi 1, Bahareh Hajikhani2, Shahin Najar Pirayeh3 and Mohammad Aghazadeh4
1Student Research Center, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran.
2Avicenna Research institute, Shaheed Beheshti University of Medical sciences, Tehran, Iran.
3Department of Medical Bacteriology, School of Medicine, Tarbiat Modares University, Tehran, Iran.
4Infectious Disease and Tropical Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Accepted 14 October, 2019
Abstract
Helicobacter Pylori is a bacillus that naturally colonizes humans, living in gastric mucus. Association of H. pylori colonization of stomach with chronic gastritis, peptic ulcer and gastric malignancies has been well documented. In this study, we aimed to find presence of H. pylori in stool of HIV infected patients by PCR. 43 patients who had confirmed HIV-infection were subjected. Specific primers for hpaA (flagellar sheath adhesin) and ureB (urea amidohydrolase), of H. pylori were designed and, presence of the genome of H. pylori investigated by PCR method. 35 of the patients (81.39%) had CD4+ count below 200 and H. pylori was found in 30 patients (69.76%). Results show from screening by H. pylori in stool of HIV-infected patients that prevalence of this bacterium in these patients is high. This prevalence is similar to prevalence of H. pylori in HIV-non infected population.
Key words: Helicobacter pylori, HIV, flagellar sheath adhesin (hpaA), urea amidohydrolase (ureB), gastric.