ISSN 2326-7275
International Journal of Anatomy and Physiology ISSN: 2326-7275 Vol. 6 (5), pp. 001-006, May, 2017. © International Scholars Journals
Full Length Research Paper
Evaluation of anti-tumor activity of water-soluble polysaccharides from Dendrobium denneanum
Yijun Fan and Aoxue Luo*
Chengdu Campus of Sichuan Agriculture University, Chengdu, PR, China.
Accepted 07 October, 2016
Abstract
Three water-soluble polysaccharides (DDP1-1, DDP2-1 and DDP3-1) were obtained from the aqueous extracts of the stems of Dendrobium denneanum by hot water extraction, ethanol precipitation, and fractionated by DEAE-cellulose ion exchange chromatography and Sephadex G-200 gel filtration chromatography. The main structure characterizations and anti-tumor activities of three fractions were evaluated in this paper. The results showed DDP1-1 with 12.50 mg/kg could significant inhibit the increment of the tumor, increase immune index of S180 mice, and also strongly promote the secretion of IL-2, TNF- and IFN- . Therefore, results of these studies demonstrated the polysaccharide DDP1-1 has strong anti-tumor and immunomodulation abilities.
Key words: Immunomodulating, Dendrobium denneanum, polysaccharide, anti-tumor.