ISSN 2756-3413
African Journal of Virology Research ISSN 3421-7347 Vol. 8 (4), pp. 001-006, April, 2014. © International Scholars Journals
Full Length Research Paper
Colonization ability of Herbaspirillum spp. B501gfp1 in sugarcane, a non-host plant in the presence of indigenous diazotrophic endophytes
Joyce Prisca NJOLOMA1*, Moriya OOTA2, Kazuhiko TAROURA2, Yuichi SAEKI1 and Shoichiro AKA
1Department of Biochemistry and Applied Bioscience, Faculty of Agriculture, University of Miyazaki, Miyazaki, 889-2192, Japan.
2Miyako Branch, Okinawa Prefectural Agricultural Experiment Station, Nishizato 2071-40, Hirara, 906-0012, Japan.
Accepted 21 August, 2013
Abstract
Inoculating sugarcane with a mixture of diazotrophic endophytic bacteria has shown that they can provide substantial amount of biologically fixed nitrogen to the plant. The genera of diazotrophic endophytes previously isolated from sugarcane have been reported associating with other non-leguminous plants showing a broad host range. This study examined the colonization ability of a wild rice isolate, Herbaspirillum spp., in sugarcane plants in the presence of indigenous endophytes using two inoculum concentrations (102 and 108 bacterial cells ml-1). Internal tissue colonization was observed in plants inoculated with both the 102 and 108 B501gfp1 bacterial cells ml-1 inoculum concentrations. However, extensive colonization and higher bacterial numbers were determined only in the basal stem tissues of plants inoculated with the 108 bacterial cells ml-1.
Key words: Sugarcane, wild rice isolate, indigenous endophytes, Herbaspirillum spp.