ISSN 2756-3669
International Journal of Bacteriology Research ISSN 2932-7521 Vol. 3 (2) pp. 096-101, February, 2015. © International Scholars Journals
Full Length Research Paper
High temperature effects on feasibility of Lactobacillus casei and examination of secreted and GroEL proteins profiles
*Lious E. Hertzog, Baleka Suzman and E. B. Gerhardus
Department of Microbiology, Faculty of Medical Sciences, University of South Africa, Pretoria, South Africa.
Email: [email protected]
Accepted 03 August, 2014
Abstract
The bacterial heat shock response is characterized by the elevated expression of a number of chaperone complexes including the GroEL and the rate change of synthesis of certain proteins (total and secreted). In this work, after incubation at 45°C, total and secreted proteins profiles of stressed bacteria were found to be altered when analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). In addition, the level expression of GroEL was evaluated with Western blot. Our results show a marked increase in both GroEL expression and protein synthesis at 45°C. These modifications were manifested by the appearance and/or disappearance of bands as well as in the level of expression of certain proteins.
Key words: Lactobacillus casei, heat shock, GroEL, secreted and total proteins, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), Western blotting.