ISSN 4391-3199
Isolation and purification of membrane-bound cytochrome c from Proteus mirabilis
Muhammad Atif Zia1*, Muhammad Shahid Nazir, Tariq Ismail and Muhammad Hassan Qureshi
Biochemistry Laboratory, Department of Chemistry, Government College University, Lahore, Pakistan.
*Corresponding authors E-mail: [email protected]
Accepted 4 April, 2013
Abstract
In the present studies, respiratory chain pathogenic bacterium, Proteus mirabilis, was investigated. In the first phase, growth profile study was performed to optimize the P. mirabilis growth. Maximum bacterial growth could be obtained between 10 – 12 h of culturing time. Down-stream processing was performed by using sonication, ultracentrifugation and detergent solubilization techniques. Partially purified respiratory contents were analyzed spectrophotometrically. Pyridine-ferrochrome and redox spectra showed the presence of heme-c.
Key words: Cytochrome c, respiratory chain and Proteus mirabilis.