ISSN 2756-3669
International Journal of Bacteriology and Mycology ISSN 2932-7521 Vol. 8 (2), pp. 001-003, February, 2019. © International Scholars Journals
Short communication
Screening of UV-irradiated and S-2-aminoethyl-L-cysteine resistant mutants of Bacillus megaterium for improved lysine accumulation
I. A. Ekwealor1* and J. A. N. Obeta2
1Department of Applied Microbiology & Brewing, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria.
2Department of Microbiology, University of Nigeria, Nsukka, Enugu State, Nigeria.
Accepted 17 November, 2018
Abstract
The screening of UV irradiated and aminoethyl-l-cysteine resistant (AECR) mutants of Bacillus megaterium strains for improved lysine accumulation was conducted. The mutants, MR-10 and MR -25 derived form irradiated cultures of B. megaterium SP 76, and MS-3 and MS-5 derived form B. megaterium SP 86 produced higher lysine levels than the parent strains. In contrast, however, lysine accumulation by MV-2 and MV-18 mutants of B. megaterium SP 14, was of low yield. In aminoethyl-l-cysteine resistant mutants, R-14 and R-76, derived from B. megaterium SP 14 and B. megaterium SP 76 respectively, lysine accumulation was enhanced.
Key words: L-Lysine, fermentation, screening, mutants, accumulation.